Effect of vaginal lubricants on sperm motility and chromatin integrity: a prospective comparative study
Objective: To evaluate the effect of vaginal lubricants Pre-Seed, FemGlide, Astroglide, and Replens on human sperm motility and chromatin integrity.
Design: Prospective, comparative, in vitro study.
Setting: Andrology laboratory at tertiary care hospital.
Patient(s): Thirteen normozoospermic donors.
Intervention(s): Semen samples from 13 subjects were incubated in human tubal fluid media (HTF) controls and 10% (vol/vol) of Pre-Seed, FemGlide, Astroglide, and Replens lubricants. After 30 minutes, progressive sperm motility was assessed by light microscopy. Semen samples of 12 patients were placed in positive control (HTF), negative control (10% K-Y Jelly lubricant), and 10% vol/vol Pre-Seed and FemGlide lubricants. After 4 hours culture, spermatozoa were analyzed for percent DNA fragmentation index with use of the acridine orange-based sperm chromatin structure assay.
Main Outcome Measure(s): Sperm motility and percent DNA fragmentation index.
Results: Percent motility did not differ significantly between HTF controls and Pre-Seed, whereas FemGlide, Replens, and Astroglide lubricants demonstrated a significant decrease in motility. There was no significant difference in percent DNA fragmentation index between the HTF controls and Pre-Seed, but a significant decline in sperm chromatin quality occurred with FemGlide and K-Y Jelly.
Conclusion: Pre-Seed does not cause a significant decrease in progressive sperm motility or chromatin integrity in contrast to other lubricants used by couples.
Abstract Source: https://www.ncbi.nlm.nih.gov/pubmed/17509584
Effect of vaginal lubricants on sperm motility and chromatin integrity: a prospective comparative study.
Agarwal A, Deepinder F, Cocuzza M, Short RA, Evenson DP.
Fertil Steril. 2008 Feb;89(2):375-9. Epub 2007 May 16.